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Zeiss Model AxioScope A.1
LED Fluorescent Illumination
Phase Contrast – Darkfield Microscopy
DAPI-FITC-TXRED LED Modules
EC Plan-Neofluar Objectives
Ergonomic Variable Height Head
Modern Product Line
Like New Condition
Upright
Fluorescence Microscope

USD $13,500.00

ITEM CODE: SKU-014
Shipping Cost: Free Worldwide Shipping

Main Features:

  • Condition is like new for this modern product line model. It appears to have little to now usage.
  • Configured with Brightfield, Darkfield, Phase Contrast, and Fluorescence Microscopy.
  • (Has the required frame that can be upgraded to DIC if needed in future.)
  • Condenser has settings for: phase (Ph1, Ph2, Ph3), Darkfield, Brightfield.
  • Numerical Aperature 1.2.
  • Includes Zeiss Centering Telescope for centering phase annuli/rings.
  • Viewing Ergonomic Trinocular head can vary height to adjust for different physical sizes of users.
  • Eyepiece pair Pl 10x / 23 with high eyepoint. These are fine for usage with or without the user having eyeglasses on.
  • Eyepieces each come with a pair of the rubber eyecups, the version for blocking out extra light from the sides. This is best for fluorescent viewing since the specimen image is of lower intensity.
  • Right hand stage with XY low position movement. With stage finger slider to hold slides.
  • Trinocular Viewing Head.
  • Has built-in camera port.
  • Camera is not included. It is only shown in the photos to demonstrate how it attaches.
  • Push/Pull Lever gives 100 percent of image to eyepieces or 100 percent to camera.
  • Includes 1x c-mount camera attachment.
  • DOES NOT INCLUDE CAMERA OR LAPTOP. Camera is shown in images to demonstrate that one can be connected.

Objective Lens and Nosepiece:

  • Six place M27 objective nosepiece with three DIC slots. With the following lenses:
  • Zeiss EC Plan Neufluor 10X/0.3 Oil PH1. List price on Zeiss website: $1578.
  • Zeiss EC Plan Neufluor 20X/0.5 Oil PH2. List price on Zeiss website: $2147.
  • Zeiss EC Plan Neufluor 40X/0.75 Oil PH2. List price on Zeiss website: $2392.
  • Zeiss EC Plan Neufluor 100X/1.3 Oil PH3. List price on Zeiss website: $4293.
  • Each nosepiece position has a blank plastic slider where DIC prisms could be inserted.

Transmitted Illumination:

  • 50W Halogen Lamp Housing and Reflector Type Bulb. Includes bulb.
  • Built-In Filter Wheel. ND 1.5 (1.5%), ND 6 (6%), ND 25 (25%). Given in percentage of transmission. These filters are installed in the base. They are selectable via a rotating knob.
  • The complete settings are: ND 1.5, ND6, ND25, complete blockage, 100 percent transmission (no filter).
  • Two filters to fit loosely as needed, on top of the Koehler field diaphragm: Light balancing daylight and blue.

Incident Light – Fluorescence Illumination:

  • LED Fluorescence Illumination.
  • No need for centering.
  • UV light shield.

Dichroic Fluorescence Filter Cubes:

    • Four-place manually rotated excitation filter wheel.
    • Includes three filter sets. They look unused with no visible wear. All performed perfectly and gave excellent imaging.
    • One filter cube space (space#1) is open. This is for use with brightfield, darfield, and phase contrast.

 

Below are the specific details on each included filter set:

  • Zeiss Filter Set 49 DAPI. (space#2)
    Item Number: 488049-9901-000
    Excitation G 365, BS FT 395, EM BP 445/50
    Zeiss Price Online USD $686. Filter set only, not including cube.
    Link to Spectral Chart: Zeiss Filter Set 49 Wavelength Chart.jpg
  • Zeiss Filter Set 38 Endow GFP shift free (E) (space#3)
    Item Number: 000000-1031-346
    Excitation Band Pass 470/40, BS FT 495, EM BP 525/50
    Zeiss Price Online USD $947. Filter set only, not including cube.
    Link to Spectral Chart: Zeiss Filter Set 38 Wavelength Chart.jpg
  • Zeiss Filter Set 45 HQ TexasRed, d=25 shift free (E) (space#4)Item Number: 000000-1114-462
    Excitation Band Pass 560/40, BS FT 585, EM BP 630/75
    Zeiss Price Online USD $941. Filter set only, not including cube.
    Link to Spectral Chart: Zeiss Filter Set 45 Wavelength Chart.jpg

LED Fluorescence Illumination Modules:

    • Capacity for up to four LED modules.
    • Includes three LED Modules.
    • LED Space #1: This is empty. It has no LED module. This empty module is in position when microscope is used for transmitted light microscopy including brightfield, darkfield and phase contrast.
    • LED Space #2: DAPI. 365nm. Zeiss Part#423052-9510.
    • LED Space #3: GFP. 470nm. Zeiss Part#423052-9570.
    • LED Space #4: N-White 540-580. Use for TexasRed. Zeiss Part#423052-9600.

 

Note: When the filter cube is selected by rotating the turret, the proper LED Illumination module is automatically engaged. So for example, if you rotate to the DAPI filter set (filter space#2), the microscope automatically engages the DAPI 365nm LED module (LED space#2).

Comments:

  • This microscope is in great condition.
  • We are including a phase centering telescope for viewing alignment of phase annulus in condenser with phase ring in objective. We are also including the needed tools for centering the phase annulus.

Quality:

  • This is a high quality Zeiss (Made in Germany) optical instrument.
  • We also sell Chinese made fluorescence microscopes but the quality is substantially lower.
  • We highly discourage buying the Chinese microscopes due to the problems that incur after the sale.

Testing Results:

  • This microscope was fully tested. Results are as follows:
  • All functions were found to be in proper working order.
  • Brightfield – Koehler illumination was set properly, with all adjustments and centering being properly achieved.
  • Phase Contrast – All objectives performed well under phase. Images achieved were good.
  • Incident Reflected Fluorescence – LED modules were turned on and resulting image had uniform illumination distribution across the complete field of view.
  • All three filters cubes were tested. All are in like-new condition with no visible wear. All performed well and obtained bright and clear images.

Optional Accessories:

  • We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
  • Fully upgradable to DIC (differential interference contrast). Contact us how.
  • Other objectives and filter cubes can be configured.

User Manual and Instructions:

Click this link:  Zeiss Axio Scope.A1 Microscope Brochure

Click this link: Zeiss Axio Scope.A1 Microscope Operating Manual


TERMS AND CONDITIONS:


Warranty Excludes Objective Lenses:
Our warranty excludes the objective lenses. There are several reasons for this. First of all, if the objective is good, it will continue good performance unless there is some user or environmental abuse. Abuse can be from oil getting on a non-oil immersion lens, contact with a solution that degrades the cement around the glass, contact with other chemicals that may stain, etch or pit the glass, scratching the lens by incorrect cleaning methods such as cloths not of lens cleaning quality, damaging the lens by scraping it on slides or metal slide holders on the stage, dropping on a hard surface, subjecting the objective to extreme humidity causing internal mold or mildew, etc.
In addition, objectives can’t feasibly be repaired. If an objective is damaged so that the optical quality is significantly diminished, it is suggested to throw it away. One solution is to send it back to the factory and incur a repair fee equal to about half the price of a new replacement. That is usually cost prohibitive. We professionally clean the front and back lens, and the outside barrel of the objective. But we do not open them up. That should only be done by the factory under strict conditions and with optical laser alignment. Generally, when someone other than the factory messes with a lens, the performance may still be acceptable, but likely will be noticeably lower quality when compared side by side with a new lens. So since defective objectives are generally thrown away, and replaced with another one, there is no need to warranty them. The user can easily find a matching objective and replace it on their own.

Objective Lenses – Condition:
All objectives have been professionally cleaned.
All are guaranteed to be free from optical defects. If any defect is found, it will be clearly noted in the ad.
Our inspection of the objective lenses includes checking for the following:
lens cracking, pitting, lens cement not intact, internal oil damage or oil seepage, glass fogging, glass hazing, stains on optical glass, mold or mildew stains, and delamination (separation of internal lenses).

Condition of Equipment:
This reconditioned microscope is complete and in excellent working order and has been completely checked out and serviced.

Technical Sales and Support:
Most offerings of used microscopes are by simple flippers of equipment who have obtained defective and rejected microscopes from labs discarding the equipment. They resell exactly what they got, as they got it, and without any technical knowledge of it. You can rest assured when purchasing from us that we are professional dealers who are well trained to service and provide technical support of the microscopy equipment. We test, repair and refurbish all equipment to excellent working conditions. We are well trained and able to give you technical advice including pre-sale and post-sale support.

Microscope Training – Setup Support – Software Assistance:
Concerned about how to set up and operate this equipment? Need training? Need help with software for the camera? We can help. Contact us for more details.

Free Application Evaluation and Recommendation:
We strongly encourage all potential buyers to first contact us with regards to their specific application. In most situations, we need to customize certain features of the microscope in order to best fit the end user’s technical requirements. We want to ensure you have the correct and optimal configuration for your application.

Fluorescence Filter Sets – Customized:
We are dealers of both Chroma and Omega brands (Made in USA) of dichroic filters. Please contact us regarding the specific fluorophores you are using so we can be sure the filter sets provided are a proper match. Incorrect matching will give you poor quality or no visible fluorescing.

Free Worldwide Shipping:
Equipment may ship from Outside USA. We provide free worldwide shipping. The method of shipping may vary, depending on destination country, but may be via commercial air cargo. Not all countries will have door to door service. Contact us for further details so we can discuss shipping arrangements.

Customs Duty and Tax:
Any and all customs brokerage, customs entry, VAT and duty/tax are the responsibility of the buyer. Buyers in USA can contact us in advance to see if there are any entry fees.

Voltage Requirement:
Our equipment may show 110V and/or 220V external power supplies. Some power supplies are not Volt selectable. Depending on your country of final destination, and the Voltage supply, we may need to change the power supply shown in the ad to one that matches your power requirements. Contact us first with your Voltage requirement so we can be certain we have the required power supply if it needs to be changed from what is shown in the ad.

Payment Method Accepted:
We generally accept purchase orders from government, educational, and industrial facilities in some countries. For some preapproved orders, we accept credit cards. We also accept bank wire (telegraphic) transfers. Contact us for details. We properly screen all buyers to protect against fraudulent transactions. We reserve the right to deny transactions deemed to be potentially fraudulent.

Financing Available for Select Customers:
If you are unable to afford the full purchase price, please contact us about financing details. Our financial partner offers 90 Days “Same as Cash” and deferred payments up to 6 Months.

Rental:
We have rental plans available for some companies and universities. Contact us for details.

Money Back Guarantee:
We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 14 days of receiving it. We will then arrange to get you details about how to return it. We have no restocking fees but the actual cost we paid for shipping will be deducted from refund amount. We give free shipping, but for returned items, this cost will be charged to buyer.

Beware of Counterfeit Fake Parts:
Be sure of whom you are buying from as a lot of “dealers” are selling counterfeit parts, passing them off as originals. Click this link.

All Original Parts – Not Counterfeit.html

Warning: UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!

Click this link.

Safety with UV Light