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Zeiss Model AxioSkop-2
Phase Contrast – Darkfield Microscopy
HBO 100W Mercury DAPI-FITC-TRITC
Low Power Scanning Lens
Upright Fluorescence Microscope

USD $8,500

ITEM CODE: SKU-007
Shipping Cost: Free Worldwide Shipping

Main Features:

  • Configured with Brightfield, Darkfield, Phase Contrast, and Fluorescence Microscopy. (Has the required frame that can be upgraded to DIC if needed in future.)
  • Achromatic Aplanatic Universal Condenser has settings for: phase (Ph1, Ph2, Ph3), Darkfield, Brightfield.
  • Numerical Aperature 0.9.
  • Condenser flip-out top lens for usage with included 2.5x and 5x low power scanning objective lenses.
  • Trinocular Viewing Head. Split image between camera and eyepieces. Has built-in camera port.
  • Eyepiece pair W-Pl 10x / 23 with high eyepoint. These are fine for usage with or without the user having eyeglasses on. Note that these are the more expensive, wider field, 23mm as compared to 18mm and 20mm.
  • One eyepiece has a measuring reticle, 10mm in 100 divisions, 0.1mm resolution. This reticle can be easily removed if desired.
  • Eyepieces each come with a pair of the rubber eyecups, the version for blocking out extra light from the sides. This is best for fluorescent viewing since the specimen image is of lower intensity.
  • Right hand stage with XY low position movement. With stage finger slider to hold slides.
  • DOES NOT INCLUDE CAMERA OR LAPTOP. Camera is shown in images to demonstrate that one can be connected.
  • With c-mount for camera attachment.

Incident Light – Fluorescence Illumination:

  • 100W HBO lamp housing and power supply.
  • UV light shield.
  • Adjusting Aid for easy alignment of Mercury Vapor Short-Arc lamp.
  • Aperature Diaphragm with centering.
  • Luminous Field Diaphragm with centering.
  • Transmitted Illumination:
  • 100W Halogen Lamp Housing and Bulb.
  • Built-In Filter Magazine. ND 1.5 (1.5%), ND 6 (6%), ND 25 (25%). Given in percentage of transmission.
  • With Diffusion disk lever to engage/disengage.
  • Quick selection – 3200K color temperature button.

Objective Lens and Nosepiece:
Five place DIC compatible objective nosepiece with the following lenses:

  • Plan-NeoFluar 2.5x / 0.075. Infinity/-. Lens for low power scanning.
  • Plan-NeoFluar 5x / 0.15. Infinity/0.17. Phase Ph1.
  • Plan-NeoFluar 10x / 0.30. Infinity/0.17. Phase Ph1.
  • Plan-NeoFluar 20x / 0.50. Infinity/0.17. Phase Ph2.
  • AchroPlan 100x /1.25 Oil. Infinity/0.17. Phase Ph3.

Each nosepiece position has a blank plastic slider where DIC prisms could be inserted.

Dichroic Fluorescence Filter Cubes:
Four-Place Manually Operated Excitation filter wheel.

  • DAPI – 360/40 nm excitation. Little to no visible wear. Great condition.
  • FITC – 470/40 nm excitation. Little to no visible wear. Great condition.
  • TRIC – 546/10 nm excitation. Little to no visible wear. Great condition.
  • Includes one filter block (with open vertical path and closed horizontal path) for use with brightfield, darkfield, and phase contrast. Blocks incident light path (HBO light). Allows transmitted light to pass.

Comments:

  • This microscope is in great condition.
  • We are including a phase centering telescope for viewing alignment of phase annulus in condenser with phase ring in objective. We are also including the needed tools for centering the phase annulus.

Quality:

  • This is a high quality Zeiss (Made in Germany) optical instrument. We also sell Chinese made fluorescence microscopes but the quality is substantially lower. We highly discourage buying the Chinese microscopes due to the problems that incur after the sale.

Testing Results:

  • This microscope was fully tested. Results are as follows:
  • All functions except the sliding of the viewing aid were found to be in proper working order.
  • The viewing aid was used to center the Mercury bulb. However, this is very hard to slide. It is suggested to simply look into the eyepieces when turning the centering knobs to get the optimal image. This is best done with one person doing the centering and another person looking into the eyepieces. You can also center the bulb with a blank sheet of paper or with a centering objective.
  • Brightfield – Koehler illumination was set properly, with all adjustments and centering being properly achieved.
  • Phase Contrast – All objectives performed well under phase. Images achieved were good.
    Incident Reflected Fluorescence – HBO Bulb was properly centered obtaining bright, clear, and uniform illumination distribution across the complete field of view.

Fluorescing Capability –

  • DAPI – This filter performed well. Obtained bright and clear images.
  • FITC – This filter performed well. Obtained bright and clear images.
  • TRITC – This filter performed well. Obtained bright and clear images.

Optional Accessories:

  • We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
  • Fully upgradable to DIC (differential interference contrast). Contact us how.
  • Other objectives can be configured.
  • Brand new replacement 100W HBO Mercury Bulbs and Halogen bulbs can be added as an option.
  • Brand new Chroma hard coated filter cubes with lifetime warranty may be added. Cost is USD $750/set for the dichroic filter (emission filter, dichroic mirror, barrier filter) and included blank cube.

Fair Market Value – Discount Price:

  • Fair Market Value USD $10,000.00.
  • Discount Price USD $8,500.00.
  • We did not find any complete comparable models for sale by others. We only found one that had one metallurgical objective on it. The price was $10,000.
  • In addition, we are including a one year warranty, money back guarantee, and free shipping that are value added additions not given by most resellers and should be added to the value.

User Manual and Instructions:

  • Upon purchase, we will email links to the user manuals / instructions.

 


TERMS AND CONDITIONS:

One Year Parts and Labor Warranty:
We warrant this Microscope to be free from defects due to normal usage in normal operating conditions for a period of one year. Camera equipment (if included) is given a three month warranty. Objective lenses are excluded from warranty.
Additional warranty on microscope may be purchased if desired.

We will repair or replace equipment at no charge, during the one year warranty period. Both parts and labor are included. Buyer is responsible for shipping costs BOTH ways, paying for shipping cost to our designated repair facility and shipping cost back to buyer. Bulbs are considered user replaceable items and not covered.

Warranty Excludes Objective Lenses:
Our warranty excludes the objective lenses. There are several reasons for this. First of all, if the objective is good, it will continue good performance unless there is some user or environmental abuse. Abuse can be from oil getting on a non-oil immersion lens, contact with a solution that degrades the cement around the glass, contact with other chemicals that may stain, etch or pit the glass, scratching the lens by incorrect cleaning methods such as cloths not of lens cleaning quality, damaging the lens by scraping it on slides or metal slide holders on the stage, dropping on a hard surface, subjecting the objective to extreme humidity causing internal mold or mildew, etc.
In addition, objectives can’t feasibly be repaired. If an objective is damaged so that the optical quality is significantly diminished, it is suggested to throw it away. One solution is to send it back to the factory and incur a repair fee equal to about half the price of a new replacement. That is usually cost prohibitive. We professionally clean the front and back lens, and the outside barrel of the objective. But we do not open them up. That should only be done by the factory under strict conditions and with optical laser alignment. Generally, when someone other than the factory messes with a lens, the performance may still be acceptable, but likely will be noticeably lower quality when compared side by side with a new lens. So since defective objectives are generally thrown away, and replaced with another one, there is no need to warranty them. The user can easily find a matching objective and replace it on their own.

Objective Lenses – Condition:
All objectives have been professionally cleaned.
All are guaranteed to be free from optical defects. If any defect is found, it will be clearly noted in the ad.
Our inspection of the objective lenses includes checking for the following:
lens cracking, pitting, lens cement not intact, internal oil damage or oil seepage, glass fogging, glass hazing, stains on optical glass, mold or mildew stains, and delamination (separation of internal lenses).

Condition of Equipment:
This reconditioned microscope is complete and in excellent working order and has been completely checked out and serviced.

Technical Sales and Support:
Most offerings of used microscopes are by simple flippers of equipment who have obtained defective and rejected microscopes from labs discarding the equipment. They resell exactly what they got, as they got it, and without any technical knowledge of it. You can rest assured when purchasing from us that we are professional dealers who are well trained to service and provide technical support of the microscopy equipment. We test, repair and refurbish all equipment to excellent working conditions. We are well trained and able to give you technical advice including pre-sale and post-sale support.

Microscope Training – Setup Support – Software Assistance:
Concerned about how to set up and operate this equipment? Need training? Need help with software for the camera? We can help. Contact us for more details.

Free Application Evaluation and Recommendation:
We strongly encourage all potential buyers to first contact us with regards to their specific application. In most situations, we need to customize certain features of the microscope in order to best fit the end user’s technical requirements. We want to ensure you have the correct and optimal configuration for your application.

Fluorescence Filter Sets – Customized:
We are dealers of both Chroma and Omega brands (Made in USA) of dichroic filters. Please contact us regarding the specific fluorophores you are using so we can be sure the filter sets provided are a proper match. Incorrect matching will give you poor quality or no visible fluorescing.

Free Worldwide Shipping:
Equipment may ship from Outside USA. We provide free worldwide shipping. The method of shipping may vary, depending on destination country, but may be via commercial air cargo. Not all countries will have door to door service. Contact us for further details so we can discuss shipping arrangements.

Customs Duty and Tax:
Any and all customs brokerage, customs entry, VAT and duty/tax are the responsibility of the buyer. Buyers in USA can contact us in advance to see if there are any entry fees.

Voltage Requirement:
Our equipment may show 110V and/or 220V external power supplies. Some power supplies are not Volt selectable. Depending on your country of final destination, and the Voltage supply, we may need to change the power supply shown in the ad to one that matches your power requirements. Contact us first with your Voltage requirement so we can be certain we have the required power supply if it needs to be changed from what is shown in the ad.

Payment Method Accepted:
We generally accept purchase orders from government, educational, and industrial facilities in some countries. For some preapproved orders, we accept credit cards. We also accept bank wire (telegraphic) transfers. Contact us for details. We properly screen all buyers to protect against fraudulent transactions. We reserve the right to deny transactions deemed to be potentially fraudulent.

Financing Available for Select Customers:
If you are unable to afford the full purchase price, please contact us about financing details. Our financial partner offers 90 Days “Same as Cash” and deferred payments up to 6 Months.

Rental:
We have rental plans available for some companies and universities. Contact us for details.

Money Back Guarantee:
We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 14 days of receiving it. We will then arrange to get you details about how to return it. We have no restocking fees but the actual cost we paid for shipping will be deducted from refund amount. We give free shipping, but for returned items, this cost will be charged to buyer.

Beware of Counterfeit Fake Parts:
Be sure of whom you are buying from as a lot of “dealers” are selling counterfeit parts, passing them off as originals. Click this link.

All Original Parts – Not Counterfeit.html

Warning: UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!

Click this link.

Safety with UV Light