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Olympus Model BX53
Upright Fluorescence Microscope
Plan Apo Objectives

DP72 Camera sold separately


MODEL: Olympus BX53
Shipping Cost: To be determined



  • Hard to find latest model upright fluorescent microscope.
  • Eight Dichroic Filter Cube Capacity in Reflector Turret.
  • If you have a huge budget, then buy it new from Olympus America Corporation. If cost is a concern, get the same thing here at a great savings. But hurry, as this will not last long. We checked all over and failed to find even one of these BX43 fluorescent upright microscopes out there for sale, outside of buying it new. We only have this one available.

Main Components:

  • BX53F Frame.
  • U-SVRB-4 Mechanical Stage with low position XY slide movement. Right hand configuration.
  • U-D6RE Six Place Sextuple Revolving Nosepiece for Objectives. Has blank slider. Useful for both DIC and simple Polarizing.
  • U-AC2 Abbe Condenser with 1.10 numerical aperature and iris diaphragm, for use with 4x to 100x objective magnifications. Note: Photos were taken with U-LC Condenser (0.75 numerical aperature)(low magnification condenser). When using the high aperature 60x and 100x objectives, the U-AC2 condenser is needed. We have included the U-AC2 instead of the U-LC.
  • U-LH100-3, 12V-100W bottom rear externally mounted transmitted illumination. Halogen bulb. This is very bright compared to the standard 30W halogen as on other models. This intense illumination makes the BX53 model ideal for darkfield microscopy and phase contrast microscopy.
  • BX3-URA Universal Reflected Illuminator. Vertical Illuminator for Reflected Fluorescence Imaging. With mirror turret.

Observation Tube:

  • U-TTR-2 Trinocular Viewing Head.
  • Ergonomic and Inclinable binoculars for ease of viewing with less neck and back strain.
  • Eyepieces: WHN10X-H/22 and WHN10X/22.
  • Photography Camera and Video Port on the top of the head.
  • Ability for all light to binoculars, all light to camera port, or split image to both.

Objective Lenses:

  • UPlanFl 4X/0.13, /-.
  • UPlanApo 10x/0.40, 0.17mm.
  • UPlanApo 20x/0.70, 0.17mm.
  • UPlanApo 40x/1.0, /0.17mm, Oil Immersion. Iris Diaphragm (N.A. 0.5-1.0).
  • PlanApo 60x/1.40, Oil Immersion. 0.17mm.
  • UPlanApo 100x/1.35, /0.17mm, Oil Immersion. Iris Diaphragm (N.A. 0.5-1.35).
  • Note: We can provide different lenses as per user’s application requirements.

Fluorescence Illuminator.

  • Prior Lumen 200. By Prior Scientific. ( click here )
  • 200W Metal Halide.
  • Up to 20 times longer bulb life than traditional HBO mercury vapor lamps, and similar spectral output.
  • None of the typical time consuming alignment of HBO with this simple fiber optic cable system.

Fluorescence Filter Cubes:

  • The turret has space for a maximum of eight cubes.
  • The microscope came with these three filter cubes plus five blank cubes.

These three filter cubes are like-new:

U-FGNA (click here)

  • Excitation filter BP540-550
  • Emission filter BA575-625
  • Dicromatic filter DM570

U-FBN (click here)

  • Excitation filter BP470-495
  • Emission filter BA510IF
  • Dicromatic filter DM505

U-FUN (click here)

  • Excitation filter BP360-370
  • Emission filter BA420IF
  • Dicromatic filter DM410

We can provide customized filter sets as per customer’s requirements. We are a dealer for Chroma. Chroma supplies Olympus with their dichroic filters.

Misc Notes:

  • Frame is marked “Made in Japan”. Has volt rating: 100-120/220-240V, 50/60Hz.
  • Includes a UV shield but it is not shown in the photos.
  • Includes LBD. Equivalent daylight balanced filter for halogen illumination.
  • Includes ND6 and ND25 Neutral density filters, selectable on bottom of frame.
  • Lots of original factory-provided printed manuals and instructions.
  • Unit comes with all needed power cords, but they are not shown in the images.
  • The condition of this instrument is like new. We didn’t even need to clean it when it arrived. We only dusted it off. It appeared to never have been used. We did add some additional UPlanApo lenses to what it already had.

History of this Instrument:

  • This instrument came from the recently shuttered Olympus Corporation’s U.S. Biotechnology Division, Olympus Biotech. All commercial operations ended there this past May 30, 2014. This fluorescent microscope was barely used. It was not discarded due to something being defective like often times happens with used laboratory equipment.
  • Read about the facility’s closure here.


  • Optional U-LC Condenser (shown in the photos, but the ad will include a U-AC2 condenser to better match for the high numerical objectives). With the U-LC comes the auxiliary lens for the U-LC condenser that must be fitted to the light collector lens when using the U-LC. A mounting tool will be included if this option is selected. The manual explains the usage and installation.
  • Optional Olympus DP72 Camera. With U-TV1XC adapter. One photo shows the DP72 camera mounted but it is not included in the price. It can be obtained separately.
  • Optional SWH10X-H/26.5 (qty 2, Super Wide Field Eyepieces)(Field width is 26.5mm instead of 22mm).
  • Optional U-POT Polarizer that fits above the field iris diaphragm, below the condenser.
  • Optional U-ANT Analyzer that fits above the nosepiece in the slider.
  • Optional U-FDICT cube. Note that this cube is not used for fluorescence.


Sales Brochure and User Manual / Instructions:

Click this link: Olympus BX53 BX63 Microscope Brochure
Click this link: Olympus BX53 Microscope Instruction Manual

Payment Method:

We generally accept purchase orders from government, educational, and industrial facilities. We also accept bank wire (telegraphic transfers) transfers. Contact us for details.

Shipping Costs:

Shipping costs vary. Please contact us and we will review your location and the cost to ship.

Money Back Guarantee:

We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 10 days of receiving it. We will then arrange to get you an RMA number and details about how to return it. Shipping costs are non-refundable. We have no restocking fees. We will refund the full price of the equipment.

Beware of counterfeit fake parts:

Be sure of who you are buying from as a lot of “used dealers” are selling counterfeit parts, passing them off as originals.

Click this link.
All Original Parts – Not Counterfeit


UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!

Click this link.
Safety with UV Light