To properly view the site, activate javascript in your browser preferences.
Leave a message
Call Us Now: or Email us at:

Zeiss Model AxioSkop
HBO 50W Mercury
Upright Fluorescence Microscope

USD $4,250

Shipping Cost: Free Worldwide Shipping


Main Features:

  • Trinocular Head with Photography Port.
  • Two position prism slider. Push slider in for 100% light to eyepieces. Pull out for 30% to eyepieces and 70% to camera port.
  • Right Hand XY stage.
  • Flip-Out Condenser, N.A. 0.9. Capable of usage with optional low power scanning lenses such as 2.5x.
  • Six place nosepiece. (Not upgradable to DIC. If you want DIC, we have other units.)
  • Made in Germany. This is the newer version of the Axioskop model.
  • Eyepiece pair Pl 10x/20 with high eyepoint for both with eyeglass usage or no eyeglasses.
  • Installed with the better cupping eyeguards to block out ambient light.
  • DOES NOT INCLUDE CAMERA OR LAPTOP. Camera is shown in images to demonstrate that one can be connected.
  • Includes C-Mount for attaching a camera.

Fluorescence Illumination:

  • Lamp housing and external Mercury power supply for 50W HBO light.
  • A brand new 50W bulb was installed. We opened it from a new package. It will be included.
  • Includes a Red Attenuating filter that helps cut out unwanted IR.
  • Includes an incident light heat-reflecting filter to help preserve the integrity of the internal optical lenses and sensitive dichroic filter cubes. This is an important filter that is often missing.

Transmitted Illumination:

  • 12V-50W Socket at rear of frame to power the included 12V-50W halogen light bulb.
  • Do not install 100W halogen bulb. Lamp housing is capable of going up to 100W but do not install higher than
  • 50W since the frame housing is rated 50W.
  • Frosted Diffusion filter for transmitted light.
  • Includes a transmitted light heat-reflecting filter.

Objective Lenses:

  • Achroplan 4x /0.10. Infinity/-. (Swing out the condenser front lens with this objective).
  • Plan-Achromat 10x / 0.25. Infinity/0.17.
  • Plan-Achromat 40x / 0.65. Infinity/0.17.
  • Achroplan 100x / 1.25 Oil Immersion. Infinity/0.17.

Dichroic Filter Slider:

  • Three position filter tray:
  • Two filter sets. DAPI and FITC.
  • Both with significant wear and recommended to be replaced.
  • Middle position has a pass through with a block on the incident light. This position is for brightfield only.


  • This microscope is in good condition.
  • Better imaging would be obtained with new filter sets and high quality objectives.
  • While it is industry standard to hype up every item like it’s the best in the world, we have to say this model compares poorly to our better more expensive models such as Axioskop-2.
  • We also noted that the design of the lamp housing is quite old. It also allows a lot of light leakage into the room.
  • This is not good since UV light is harmful. This is not an issue with only this particular microscope but is an issue with the old design of the lamp housing. Newer models are much better. I would recommend this model only if your budget will not allow a more expensive unit.


  • This is a quality Zeiss (Made in Germany) optical instrument. We also sell Chinese made fluorescence microscopes but the quality is substantially lower. We highly discourage buying the Chinese microscopes due to the problems that incur after the sale. Even though this model is several years old and a lower grade “Zeiss” model, it is still a superior quality to a brand new Chinese made.

Testing Results:

This microscope was fully tested. Results are as follows:
All functions were found to be in proper normal working order.

  • Brightfield – Koehler illumination was set properly, with all adjustments and centering being properly achieved.
  • Incident Reflected Fluorescence – HBO Bulb was centered reasonably well, but maybe not good enough. The fluorescing image was only obtained over a little more than half of the field of view. Imaging is fine in brightfield. So it has something to do with the bulb not being centered.

Fluorescing Achieved –

  • FITC filter set – obtained good results using 40x. Bad using 4x and 10x.
  • DAPI filter set – obtained good results using 40x. Bad using 4x and 10x.
  • Both filter sets need replaced with new.

Optional Accessories:

  • We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
  • Capable of Phase Contrast Microscopy.
  • Optional DIC configuration.
  • Other objectives can be configured.
  • We can provide brand new replacement 50W HBO Mercury Bulbs and Halogen bulbs.
  • Brand new Chroma hard coated filter cubes with lifetime warranty may be added. Cost is USD $450/set for the dichroic filter (emission filter, dichroic mirror, barrier filter).

Fair Market Value – Discount Price:

  • Fair Market Value USD $4,800.00.
  • Discount Price USD $4,250.00.
  • We found one unit for $12,000 but it has DIC which ours does not.
  • We found one at $1800 called “fluorescence” but really wasn’t, and has no components for such.
  • Another for $3000, and same, had no fluor components.

When comparing our price to others, keep in mind these things:
We are including a one year warranty, money back guarantee, and free shipping that are value added additions not given by most resellers and should be added to the value.

User Manual and Instructions:
Click this link: Zeiss Axioskop Transmitted and Incident Light Fluorescence Instruction Manual
Click this link: Zeiss HBO 50 – HBO 100 – XBO 75 Installation and Alignment Manual


One Year Parts and Labor Warranty:
We warrant this Microscope to be free from defects due to normal usage in normal operating conditions for a period of one year. Camera equipment (if included) is given a three month warranty. Objective lenses are excluded from warranty.
Additional warranty on microscope may be purchased if desired.

We will repair or replace equipment at no charge, during the one year warranty period. Both parts and labor are included. Buyer is responsible for shipping costs BOTH ways, paying for shipping cost to our designated repair facility and shipping cost back to buyer. Bulbs are considered user replaceable items and not covered.

Warranty Excludes Objective Lenses:
Our warranty excludes the objective lenses. There are several reasons for this. First of all, if the objective is good, it will continue good performance unless there is some user or environmental abuse. Abuse can be from oil getting on a non-oil immersion lens, contact with a solution that degrades the cement around the glass, contact with other chemicals that may stain, etch or pit the glass, scratching the lens by incorrect cleaning methods such as cloths not of lens cleaning quality, damaging the lens by scraping it on slides or metal slide holders on the stage, dropping on a hard surface, subjecting the objective to extreme humidity causing internal mold or mildew, etc.
In addition, objectives can’t feasibly be repaired. If an objective is damaged so that the optical quality is significantly diminished, it is suggested to throw it away. One solution is to send it back to the factory and incur a repair fee equal to about half the price of a new replacement. That is usually cost prohibitive. We professionally clean the front and back lens, and the outside barrel of the objective. But we do not open them up. That should only be done by the factory under strict conditions and with optical laser alignment. Generally, when someone other than the factory messes with a lens, the performance may still be acceptable, but likely will be noticeably lower quality when compared side by side with a new lens. So since defective objectives are generally thrown away, and replaced with another one, there is no need to warranty them. The user can easily find a matching objective and replace it on their own.

Objective Lenses – Condition:
All objectives have been professionally cleaned.
All are guaranteed to be free from optical defects. If any defect is found, it will be clearly noted in the ad.
Our inspection of the objective lenses includes checking for the following:
lens cracking, pitting, lens cement not intact, internal oil damage or oil seepage, glass fogging, glass hazing, stains on optical glass, mold or mildew stains, and delamination (separation of internal lenses).

Condition of Equipment:
This reconditioned microscope is complete and in excellent working order and has been completely checked out and serviced.

Technical Sales and Support:
Most offerings of used microscopes are by simple flippers of equipment who have obtained defective and rejected microscopes from labs discarding the equipment. They resell exactly what they got, as they got it, and without any technical knowledge of it. You can rest assured when purchasing from us that we are professional dealers who are well trained to service and provide technical support of the microscopy equipment. We test, repair and refurbish all equipment to excellent working conditions. We are well trained and able to give you technical advice including pre-sale and post-sale support.

Microscope Training – Setup Support – Software Assistance:
Concerned about how to set up and operate this equipment? Need training? Need help with software for the camera? We can help. Contact us for more details.

Free Application Evaluation and Recommendation:
We strongly encourage all potential buyers to first contact us with regards to their specific application. In most situations, we need to customize certain features of the microscope in order to best fit the end user’s technical requirements. We want to ensure you have the correct and optimal configuration for your application.

Fluorescence Filter Sets – Customized:
We are dealers of both Chroma and Omega brands (Made in USA) of dichroic filters. Please contact us regarding the specific fluorophores you are using so we can be sure the filter sets provided are a proper match. Incorrect matching will give you poor quality or no visible fluorescing.

Free Worldwide Shipping:
Equipment may ship from Outside USA. We provide free worldwide shipping. The method of shipping may vary, depending on destination country, but may be via commercial air cargo. Not all countries will have door to door service. Contact us for further details so we can discuss shipping arrangements.

Customs Duty and Tax:
Any and all customs brokerage, customs entry, VAT and duty/tax are the responsibility of the buyer. Buyers in USA can contact us in advance to see if there are any entry fees.

Voltage Requirement:
Our equipment may show 110V and/or 220V external power supplies. Some power supplies are not Volt selectable. Depending on your country of final destination, and the Voltage supply, we may need to change the power supply shown in the ad to one that matches your power requirements. Contact us first with your Voltage requirement so we can be certain we have the required power supply if it needs to be changed from what is shown in the ad.

Payment Method Accepted:
We generally accept purchase orders from government, educational, and industrial facilities in some countries. For some preapproved orders, we accept credit cards. We also accept bank wire (telegraphic) transfers. Contact us for details. We properly screen all buyers to protect against fraudulent transactions. We reserve the right to deny transactions deemed to be potentially fraudulent.

Financing Available for Select Customers:
If you are unable to afford the full purchase price, please contact us about financing details. Our financial partner offers 90 Days “Same as Cash” and deferred payments up to 6 Months.

We have rental plans available for some companies and universities. Contact us for details.

Money Back Guarantee:
We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 14 days of receiving it. We will then arrange to get you details about how to return it. We have no restocking fees but the actual cost we paid for shipping will be deducted from refund amount. We give free shipping, but for returned items, this cost will be charged to buyer.

Beware of Counterfeit Fake Parts:
Be sure of whom you are buying from as a lot of “dealers” are selling counterfeit parts, passing them off as originals. Click this link.

All Original Parts – Not Counterfeit.html

Warning: UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!

Click this link.

Safety with UV Light