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Zeiss AxioVert 200
Inverted Fluorescence
Phase Contrast DIC Varel Relief Microscope

USD $14,000

Model: Axiovert 200
Shipping Cost: Free USA Shipping.


Main Features:

  • Loaded configuration. This unit has been set up with some of the best options.
  • Specifically configured for specimen vessels with a typical 1 mm thickness. The 40x objective has a correction collar for other thicknesses.  Not configured for high power magnification.
  • Configuration has excellent capability to image using normal size and larger specimen vessels. It can handle large vertical size specimen vessels. The complete optical system is for long working distance.
  • Depending on your application, this system may or may not be the best for you. It is recommended that you contact us first, telling us specifically what you are trying to image.


DIC – Differential Interference Contrast Microscopy:

  • The 40x objective has been configured for DIC.
  • It can be beneficial to acquire DIC images simultaneously with fluorescence images since it can provide useful morphological information.


VAREL – Variable Relief Contrast Microscopy:

  • This is another excellent way of examining live cells in culture vessels.
  • It includes three illumination modes: unilateral darkfield, VAREL (variable relief) contrast, and inclined brightfield.  This microscopy technique has many advantages such as being able to examine specimens in culture vessels with a curved bottom. It also makes it possible to contrast cells near the edges of the holes in microtiter plates.  The method is an excellent way to image low contrast specimens in ways not seen with normal phase contrast or DIC.


Phase Contrast Microscopy:

  • All objectives are phase contrast.
  • Condenser has the following phase contrast annuli:  Ph0, Ph1, Ph2.


Fluorescence Microscopy:

  • The reflector turret has spaces for five cubes.
  • This has installed: DAPI, FITC, and TRITC dichroic filter cubes. They all produced good fluorescence images.
  • FITC – Zeiss filter Set Number 09. Ex. BP 450-490, FT510, LP515.
  • DAPI – Zeiss filter Set Number 02. Ex. G365, FT 395, Em. LP 420.
  • TRITC – Zeiss filter Set Number 15. Ex. BP 546/12, FT 580, Em. LP 590.
  • Includes two blank blockers for the empty spaces. These are important to protect your eyes from damaging UV light.


Metal Halide Exfo X-Cite 120 Florescent Illuminator: 

  • This type of illuminator does not need to have any bulb adjustment.
  • It has a liquid light guide (brand new), a used bulb and a collimator included.
  • Manual Frame: (Not motorized).
  • The manual versions are rare. They are highly sought after because of their ease of usage and significantly less maintenance issues. On a motorized system, everything is controlled electronically and has to be programmed. These are prone to failure.


Objective Lenses:

  • Six place objective nosepiece.
  • 10x:  A-Plan 10x / 0.25, Infinity/-. Ph1, VAR1. Phase Contrast. Variable Relief Contrast.
  • 20x: LD A-Plan 20x / 0.30, Infinity/1.0. Ph1, VAR1. Phase Contrast. Variable Relief Contrast.
  • 32x: LD A-Plan 32x / 0.40, Infinity/1.0. Ph1, VAR1. Phase Contrast. Variable Relief Contrast.
  • 40x: LD AchroPlan 40x / 0.60, Infinity/0-2. Ph2. Phase Contrast. Correction Collar.
  • Note: The 10x, 20x, and 32x objectives are not for fluorescence.
  • The 40x will work fine in fluorescence but it is not the best. We recommend you email us your specific application and we can make a recommendation for additional objectives.


General Specifications:

  • High power 100W halogen illumination. Compare this to the simple 30W versions. The 100W is superior for phase, VAREL variable relief contrast, and DIC.
  • LCD display shows the current microscope objective and illumination settings.  We have programmed the light manager system to display which objective is engaged. We have also programmed it so the light intensity is lower for the 10x so you do not hurt your eyes.
  • Left side camera port.  100 percent to eyepieces, 100  percent to camera, or split to eyepieces and camera.
  • Electronic shutter to immediately cut the fluorescence light.  Also includes manual shutter slider.
  • Includes IR cut-off heat reflecting filter to protect internal lenses from the extreme heat.
  • Eyepieces: W-PL 10 X/23
  • Autosensing voltage: 100…240 VAC. 50…60 Hz.
  • Two carrying handles.
  • Travel focus lock stop (manually set).
  • Six – place nosepiece.
  • Field Aperture and Field Diaphragm sliders.
  • Does not include a camera or c-mount. That is only shown to demonstrate how one can be connected.


Mechanical XY Stage:

  • This is a premium grade extra wide stage.
  • This whole stage moves.
  • Compare this to simple platform stages that have a sliding part that holds the vessel adapter and slides over the stage.
  • XY controls are located on the right hand side.
  • Installed right hand extra wide mechanical stage.
  • The multi-use stage adapter plate holds glass slides as well as petri dishes and other specimen vessels. It can hold a petri dish that is up to 83 mm diameter.


Buy with Confidence:

  • Note: Unlike others who often describe equipment as being DIC, we actually test our equipment.
  • We obtained high quality differential interference contrast images with our configuration.
  • Oftentimes, mismatched or improperly configured DIC systems may initially appear to be correct until you try to get an actual image showing relief contrast. We were able to obtain textbook quality images. Anything less to us is unacceptable and indicative of improperly functioning equipment.



  • This microscope is in great condition.
  • Includes all the tools for proper assembly and disassembly.
  • This is a high quality Olympus (Made in Germany) optical instrument.
  • We have a wide variety of optional scientific cameras, both monochrome and color, with and without Peltier cooling.
  • Upon purchase, we will email links to the user manuals / instructions.
  • When comparing prices, keep in mine the extras that we are providing.
  • We are including a one year warranty, money back guarantee, and free shipping that are value added additions not given by most resellers.



One Year Parts and Labor Warranty:
We warrant this Microscope to be free from defects due to normal usage in normal operating conditions for a period of one year. Camera equipment (if included) is given a three month warranty. Objective lenses are excluded from warranty.
Additional warranty on microscope may be purchased if desired.

We will repair or replace equipment at no charge, during the one year warranty period. Both parts and labor are included. Buyer is responsible for shipping costs BOTH ways, paying for shipping cost to our designated repair facility and shipping cost back to buyer. Bulbs are considered user replaceable items and not covered.

Warranty Excludes Objective Lenses:
Our warranty excludes the objective lenses. There are several reasons for this. First of all, if the objective is good, it will continue good performance unless there is some user or environmental abuse. Abuse can be from oil getting on a non-oil immersion lens, contact with a solution that degrades the cement around the glass, contact with other chemicals that may stain, etch or pit the glass, scratching the lens by incorrect cleaning methods such as cloths not of lens cleaning quality, damaging the lens by scraping it on slides or metal slide holders on the stage, dropping on a hard surface, subjecting the objective to extreme humidity causing internal mold or mildew, etc.
In addition, objectives can’t feasibly be repaired. If an objective is damaged so that the optical quality is significantly diminished, it is suggested to throw it away. One solution is to send it back to the factory and incur a repair fee equal to about half the price of a new replacement. That is usually cost prohibitive. We professionally clean the front and back lens, and the outside barrel of the objective. But we do not open them up. That should only be done by the factory under strict conditions and with optical laser alignment. Generally, when someone other than the factory messes with a lens, the performance may still be acceptable, but likely will be noticeably lower quality when compared side by side with a new lens. So since defective objectives are generally thrown away, and replaced with another one, there is no need to warranty them. The user can easily find a matching objective and replace it on their own.

Objective Lenses – Condition:
All objectives have been professionally cleaned.
All are guaranteed to be free from optical defects. If any defect is found, it will be clearly noted in the ad.
Our inspection of the objective lenses includes checking for the following:
lens cracking, pitting, lens cement not intact, internal oil damage or oil seepage, glass fogging, glass hazing, stains on optical glass, mold or mildew stains, and delamination (separation of internal lenses).

Condition of Equipment:
This reconditioned microscope is complete and in excellent working order and has been completely checked out and serviced.

Technical Sales and Support:
Most offerings of used microscopes are by simple flippers of equipment who have obtained defective and rejected microscopes from labs discarding the equipment. They resell exactly what they got, as they got it, and without any technical knowledge of it. You can rest assured when purchasing from us that we are professional dealers who are well trained to service and provide technical support of the microscopy equipment. We test, repair and refurbish all equipment to excellent working conditions. We are well trained and able to give you technical advice including pre-sale and post-sale support.

Microscope Training – Setup Support – Software Assistance:
Concerned about how to set up and operate this equipment? Need training? Need help with software for the camera? We can help. Contact us for more details.

Free Application Evaluation and Recommendation:
We strongly encourage all potential buyers to first contact us with regards to their specific application. In most situations, we need to customize certain features of the microscope in order to best fit the end user’s technical requirements. We want to ensure you have the correct and optimal configuration for your application.

Fluorescence Filter Sets – Customized:
We are dealers of both Chroma and Omega brands (Made in USA) of dichroic filters. Please contact us regarding the specific fluorophores you are using so we can be sure the filter sets provided are a proper match. Incorrect matching will give you poor quality or no visible fluorescing.

Free Worldwide Shipping:
Equipment may ship from Outside USA. We provide free worldwide shipping. The method of shipping may vary, depending on destination country, but may be via commercial air cargo. Not all countries will have door to door service. Contact us for further details so we can discuss shipping arrangements.

Customs Duty and Tax:
Any and all customs brokerage, customs entry, VAT and duty/tax are the responsibility of the buyer. Buyers in USA can contact us in advance to see if there are any entry fees.

Voltage Requirement:
Our equipment may show 110V and/or 220V external power supplies. Some power supplies are not Volt selectable. Depending on your country of final destination, and the Voltage supply, we may need to change the power supply shown in the ad to one that matches your power requirements. Contact us first with your Voltage requirement so we can be certain we have the required power supply if it needs to be changed from what is shown in the ad.

Payment Method Accepted:
We generally accept purchase orders from government, educational, and industrial facilities in some countries. For some preapproved orders, we accept credit cards. We also accept bank wire (telegraphic) transfers. Contact us for details. We properly screen all buyers to protect against fraudulent transactions. We reserve the right to deny transactions deemed to be potentially fraudulent.

Financing Available for Select Customers:
If you are unable to afford the full purchase price, please contact us about financing details. Our financial partner offers 90 Days “Same as Cash” and deferred payments up to 6 Months.

We have rental plans available for some companies and universities. Contact us for details.

Money Back Guarantee:
We guarantee you will be satisfied with your purchase. If in the unlikely event you are not satisfied and want to return your item, please contact us within 14 days of receiving it. We will then arrange to get you details about how to return it. We have no restocking fees but the actual cost we paid for shipping will be deducted from refund amount. We give free shipping, but for returned items, this cost will be charged to buyer.

Beware of Counterfeit Fake Parts:
Be sure of whom you are buying from as a lot of “dealers” are selling counterfeit parts, passing them off as originals. Click this link.

All Original Parts – Not Counterfeit.html

Warning: UV light and Mercury Bulbs are Dangerous! YOU MUST Read BEFORE purchasing!

Click this link.

Safety with UV Light